Selasa, 30 Oktober 2012

Protista

Some members of Kingdom Protista are unicellular, others are colonial, and yet others are multicellular. Note that in the colonial forms, all the cells are similar with similar, generalized functions, whereas in the truly multicellular species, the “body” of the organism consists of a variety of types of cells, each type with its own specialized function. These organisms are all eukaryotes (they have a true nucleus). They all need some kind of a water-based environment--which can be fresh or marine water, snow, damp soil, polar bear hairs--in which to live. All are aerobic and have mitochondria to do cellular respiration, and some have chloroplasts and can do photosynthesis. Most of them reproduce or grow by mitosis, and some reproduce by meiosis and fertilization. Many can form cysts in adverse conditions. Protists are a major component of plankton.
Protists are grouped into three major, unofficial categories based on means by which they obtain nutrition. These are the Protozoa, the Algae, and the Fungus-like Protists. For some reason, botanists use the word “Division” to mean the same taxonomic level as “Phylum”, and since, way back everything was lumped in as either a plant or an animal, taxonomists who study Kingdom Protista (and those who study Kingdom Fungi) also still use the word “Division” to mean “Phylum”, so for example, when “Division Rhizopoda” is listed below, that means the same thing as saying “Phylum Rhizopoda”.

Protozoa:

These protists are animal-like, especially in their nutrition. They ingest their food by phagocytosis. Some have mouth-like structures into which the prey is put while others use pseudopodia to move and to engulf prey. Typical prey include bacteria and other smaller one-celled organisms.
Division Rhizopoda:
Click here for Amoeba Movie An example of a member of this Division is genus Amoeba, a fresh-water dweller. Protists in this group are unicellular and have pseudopodia. Some secrete shells around themselves, while others do not. None of them have flagella, cilia, or meiosis. Entamoeba histolytica is a parasitic form that causes amoebic dysentery. These colonize the colon and feed on bacteria, causing symptoms that range from mild diarrhea to dysentery. Typically periods of watery diarrhea, often containing blood, may alternate with constipation, and often there is flatulence and abdominal cramping. Entamoeba can be directly spread (anal sex), or indirectly spread (by drinking contaminated water). Fresh fruits and vegetables may be unsafe if fertilized with human feces, watered with contaminated water, or prepared by a person with it on his/her hands.
Division Apicomplexa:
These are all parasites and form tiny, infectious spores. All have complex life cycles. An example is Plasmodium vivax, which causes malaria, for which certain species of mosquitoes are the secondary host. It is also possible to become infected with Plasmodium parasites from a transfusion from an infected person or if a drug addict shares a syringe with an infected person. One stage in this complicated life cycle grows in the mosquito, the next stage in the newly-infected person’s liver, and the next stage invades the person’s red blood cells, rupturing the RBCs as the parasites leave to invade other cells. Symptoms include cyclical alternating chills, fever, and sweating which at first, can be mistaken for flu. While usually less than 1% of the RBCs are infected, often malaria causes anemia due to the smaller number of RBCs. Often the spleen and liver become enlarged as they try to deal with the dying RBCs. Malaria is treated with extract from the quinine tree. Remember that people with sickle-cell are more resistant because when a malaria parasite enters a RBC, the RBC sickles, killing the parasite, thereby preventing it from multiplying and spreading.
Division Zoomastigophora:
Trypanosoma This Division contains some organisms which are free-living, others which are symbionts, and yet others which are parasites. An example of a symbiotic member of this Division is the protozoans which live in the gut of termites and digest cellulose in the wood the termites eat. An example of a parasitic form would be Trypanosoma gambiense, which causes African sleeping sickness and is spread by the bite of the tsetse fly. Symptoms include irregular fever, general swelling of the lymph nodes, skin eruptions, and areas of painful local swelling. Eventually CNS symptoms like tremors, headache, apathy, and convulsions appear and become worse, leading to eventual coma and death. Early on, the parasites are found in blood and lymph, but later only in the person’s cerebrospinal fluid.
Division Ciliophora:
Click here for Paramecium Movie An example of an organism in this Division is Paramecium. These protozoans are solitary, fresh water organisms and use cilia to move. They have probably the most complex structure and organization of all cells. Rather than one nucleus, they have a larger macronucleus and several smaller micronuclei. They use a form of sexual reproduction called conjugation in which some of the micronuclei are exchanged between the two individuals involved.

Algal Protists

These protists are photosynthetic; their nutrition is plant-like. Almost all of them have chlorophyll A, most have chlorophyll C, but only a few have chlorophyll B. They also have a variety of carotenoids and other pigments, and frequently they are grouped into Divisions based on similarities in pigments.
Division Dinoflagellata:
These are abundant in plankton, occasionally occurring in large numbers. They can occasionally become so numerous that the water looks red, thus this algal bloom (meaning there are large numbers of them, having nothing to do with flowers, which they do not have) is called Red Tide. Because Dinoflagellates are toxic to humans, it is not safe to eat “shellfish” (clams, etc.) collected where Red Tide is occurring (the Protists get inside the clam shell and cannot be easily removed). Dinoflagellates are bioluminescent, that is, they are able to produce light like lightening bugs, and at night during Red Tide, the crests of the ocean waves appear to glow in the dark.
Division Euglenophyta:
Click here for Euglena Movie Probably the best-known example of this Division is genus Euglena. Each of these organisms has a flagellum on its anterior end, and this is used to propel the organism. They have chloroplast and, when in the light, do photosynthesis. If they are not in the light, they can also obtain nutrition by phagocytosis. To help them sense light (which they then move toward), Euglena have a light-sensitive “eyespot” or stigma near their anterior ends. This is not a true eye, in that it cannot do any image formation, but rather it is a photoreceptor which senses the light level in the organism’s environment.
Division Chlorophyta:
These protists are also known as the “green algae.” Their chloroplasts and the pigments therein are similar to plants (this is about the only group of algae with chlorophyll B), thus it is thought that the green algae may be the evolutionary ancestors of plants. Various species of green algae may be found in a variety of environments including both fresh and salt water, damp soil, the surface of snow, and within other organisms (lichens, hydra, polar bear hair).
Click here for Chlamydomonas Movie Chlamydomonas are unicellular and contain an eyespot (stigma), a chloroplast, two flagella, and a nucleus.
Click here for Volvox Movie Volvox are colonial and often contain darker green daughter colonies inside. Each cell posesses two flagella, enabling the colony to be mobile. There is an intercellular matrix holding the colony of cells together.
Ulva is called Sea Lettuce. This is truely multicellular, with a division of labor among the various cells, and is macroscopic. The “body” is two cells thick, and there is a specially-modified “holdfast” to anchor the organism to the ocean floor. Its life cycle includes both 1n and 2n stages (see below).
Click here for Closterium Movie Closterium is a member of the sub-group called the Desmids. Some desmids form colonies, but Closterium is solitary. Its nucleus is in the center with a cone-shaped chloroplast on each side. Each chloroplast contains a series of starch-storage organelles called pyrenoids In living Closterium, each end of the cell bears a small vacuole containing several gypsum grains which “dance” by Brownian motion.
Click here for Spirogyra Movie Spirogyra are colonial, being organized into long filaments. Each cell contains a spiral chloroplast with pyrenoids (used to store starch) and a nucleus. They have conjugation--a type of sexual reproduction in which the contents of the male gamete cell go over into the female cell.

Alternation of Generations Many green algae, especially the multicellular ones, have both sexual and asexual stages in their life cycles, thus we must re-introduce the idea of Alternation of Generations we discussed along with meiosis. When we first discussed Alternation of Generations, we looked at a very simple diagram in which adults produced 1n gametes by meiosis, and those gametes joined by syngamy to form a new 2n generation. In reality in algae and plants, there are a few more stages in the process, thus we now need to re-visit this cycle. The 2n generation, which in humans is called an “adult,” in algae and plants is called a sporophyte because it produces spores. Within specialized reproductive structures in/on the bodies of the sporophyte, meiosis occurs to reduce the chromosome number from 2n to 1n, thus the spores which are produced are 1n. Each spore germinates and grows into a new, independent, 1n organism (which often looks totally different than the 2n generation). These 1n organisms are called gametophytes because they produce the gametes (eggs and sperm), which are still 1n. An egg and sperm unite by syngamy increasing the chromosome number from 1n to 2n, and forming a zygote which is 2n. The zygote grows into the sporophyte, and the cycle starts over. Various of the green algae go through this cycle as do members of the next two groups, the brown and red algae. Plants also go through this same cycle with some interesting modifications we will discuss later.
Division Phaeophyta:
These organisms are commonly known as the “brown algae.” They are multicellular and live in marine, temperate zone, costal areas. They all have a form of sexual reproduction with alternation of generations. One member of this Division with which you may be familiar is Kelp, which actually can be any of several species of seaweed in the genera Fucus and/or Laminaria. Brown algae are used in many cultures as human food, and are good sources of iodine. We need iodine for our thyroid glands, and if a person doesn’t enough iodine in his/her diet (most commonly in inland areas where iodine is not added to salt), the thyroid gland enlarges in an attempt to keep making enough thyroid hormone (which doesn’t do any good because what it’s lacking is the iodine needed to make the hormone). This enlarged thyroid is called a goiter. Laminaria also has an interesting gynecological use. If a woman is scheduled for some medical procedure for which the doctor needs access to the inside of her uterus, often a day or so beforehand, rolled-up, dried pieces of Laminaria are inserted into the opening of the woman’s cervix. As the seaweed absorbs water from her body fluids, it gently and slowly expands, gradually stretching the cervix. Thus, by the time her surgery is scheduled, her cervix has been dilated slowly and gently rather than the doctor having to forcibly and quickly (thus painfully) stretch the cervix open minutes beforehand.
Division Rhodophyta:
These are called the “red algae.” They also are multicellular and marine-dwelling, but are more typically found in tropical zones and deeper in the ocean. They also go through alternation of generations, Many of these (such as the Nori used in sushi) are used by humans as food, and are also good sources of iodine.

Fungus-like Protists

Division Myxomycota:
These organisms are called “slime molds.” They are fungus-like in their nutrition in that they absorb nutrients from their environment. Their “body” structure is unusual in that the nuclei undergo mitosis, but there is no cytokinesis--there are no individual cells with one nucleus each. Rather, the “body” is a giant, multinucleate mass of cytoplasm. Slime molds are mobile: they move by amoeboid movement, in other words, like a giant Amoeba with giant pseudopodia. They live in decayed wood and move around in between the fibers, ingesting bacteria, etc. by phagocytosis. Slime molds are often brightly-colored (yellow or orange).
 
 
 
http://biology.clc.uc.edu/courses/bio106/protista.htm 
 

Plant Issue Culture

In 1965, French botanist George Morel was attempting to obtain a virus-free orchid plant when he discovered that a millimetre-long shoot could be developed into complete plantlets by micropropagation. This was the beginning of tissue culture. Thereafter, in the 1970s developed countries began commercial exploitation of this technology. It entered the developing world in the 1980s. It was earlier used to develop ornamental plants and flowering plants for export. With tree species, the technique of tissue culture remained confined for many years to the laboratory stage and had generally invited only academic interest. But in most developing countries, the shortage of biomass and the ever-increasing energy requirements created the need to explore possibilities of mass propagation of trees by tissue culture.
Tissue culture or mass cloning methods of elite tree species is done for increasing land productivity. They are being modified or adapted for large-scale modification.
Species are selected for tissue culture on the following basis.
bullet.gif (62 bytes) Species that have regeneration problems, specially because of poor seed set or germination (as in Anogeissus and bamboo). In these cases, seeds collected from superior trees are used for initiating cultures.
bullet.gif (62 bytes) Species that vary markedly in their desirable traits, i.e. Eucalyptus. The selected trees are marked from the variant population for the desirable trait such as disease resistance, straight bole, higher productivity, etc. in consultation with officials from state forest department or growers.
bullet.gif (62 bytes) Species where plants of any one particular sex is of commercial importance, for example female plants of papaya and male plants of asparagus
In tissue culture cells, tissues, and organs of a plant are separated. These separated cells are grown especially in containers with a nutrient media under controlled conditions of temperature and light. The cultured plant requires a source of energy from sugar, salts, a few vitamins, amino acids, etc. that are provided in the nutrient media. From these cultured parts, an embryo or a shoot bud may develop, which then grows into a whole new plantlet. Similarly, portions of organs or tissues can be cultured in a culture media. Generally, these give rise to an unorganized mass of cells called callus (soft tissue that forms over a cut surface).
Tissue culture plantlets have poor photosynthesis efficiency and lack the proper mechanism to control water loss. They need to be hardened gradually by moving them along a humidity gradient in the greenhouse. Once these plants are in the research fields, they are evaluated under field conditions and the data is collected every 6 months. A large number of tissue culture plants that have grown into trees are remarkably uniform and show an increase in biomass production over the conventionally raised plants.
Tissue culture
                                            Figure Tissue culture and totipotency
Application of tissue culture
bullet.gif (62 bytes)Micropropagation
Rapid vegetative multiplication of valuable plant material for agriculture, horticulture, and forestry.
bullet.gif (62 bytes)Production of disease-free plants
When the apex of shoot is used for multiplication by tissue culture, we get disease free plants because the shoot apical meristem, a group of dividing cells at the tip of a stem or root, is free from pathogens.
bullet.gif (62 bytes)Plant breeding
Tissue culture has also been successfully used in plant breeding programmes.
bullet.gif (62 bytes)Production of disease- and pest-resistant plants
Plants grown from tissue culture usually pass trough callus phase and show many variations. These show some agronomic characteristics like tolerance to pests, diseases, etc.
Cloning

Genetically identical plants derived from an individual are called clones. Processes that produce clones can be put under the term ‘cloning’. This includes all the methods of vegetative propagation such as cutting, layering, and grafting. Propagation by tissue culture also helps in producing clones. Using the shoot tip, it is possible to obtain a large number of plantlets. This technique is used extensively in the commercial field for micropropagation of ornamental plants like chrysanthemum, gladiolus, etc. and also crops such as sugar cane, tapioca, and potato. Thus an unlimited number of plants that are genetically similar or are clones can be produced in a short span of time by tissue culture.

Large-scale propagation
To bridge the gap between research and application, the Department of Biotechnology, Government of India sponsored the setting-up of two pilot-scale facilities for large-scale propagation of elite planting material of forest trees through tissue culture. One of these facilities has been established at TERI’s 36-hectare-campus in Gual Pahari, Haryana with an annual capacity of a million plantlets. Research at these facilities focuses exclusively on developing new protocols for mass cloning of elite planting material, mainly of trees.
Till date, over 4 million plants have been dispatched for field plantation from these facilities. The tissue culture raised plants are presently being evaluated under field conditions. This is being done in tandem with the forest departments of Haryana, Uttar Pradesh, Madhya Pradesh, Bihar, Jammu and Kashmir, and Orissa. For initial screening for phenotypically superior trees only a few hundred plantlets of the same are raised and tested under various agroclimatic zones. The best clones are then mass multiplied and monitored regularly for their performance. Field data suggest a survival percentage of more than 90% even in the harsh conditions of Aravalis without the life-saving irrigation. At half the rotation age some of the selected clones of Eucalyptus are showing a significant increase in productivity as compared to the conventional seed raised progenies.

Kamis, 25 Oktober 2012

TANGAN ILAHI

Lirik lagu nasyid :)

Mulki &Reni - Tangan Ilahi


Kasihmu tlah pergi
Cintamu tak kutemui lagi
Ternyata kau harus pergi

Mengiring Tangan Ilahi
Dan ku disini ikhlas memilih
Mendo’akanmu ibu

Memang tak ku pungkiri
Ku pasti kan merasa sedih
Tapi ku yakin ini Tangan Ilahi

Mengiring Tangan Ilahi
Dan ku disini ikhlas memilih
Mendo’akanmu ibu

Bridge : Semoga kau disana tenang dalam dekapNya
           Semoga kau disana mendapatkan pintu syurgawi yang dijanjikan..

Reff : Tangan Ilahi memberi kekuatan hati
         Tangan Ilahi membuat disini
         Tangan Ilahi memberi arti cinta sejati
         Sehingga ku ikhlas melepasmu ibu..

Minggu, 21 Oktober 2012

Do'aku Untukmu

Di depanmu..
Tak kuasa ku menatapmu berlama-lama
Karena diriku sungguh malu
Tapi, di belakangmu..
Ku berdo'a kepada-Nya berlama-lama
Untuk meminta dirimu

Rabb..
Jika Engkau ciptakan air matanya
jadikanlah tanganku tuk hapuskan kesedihannya..
Jika Engkau ciptakan peluhnya
jadikanlah jiwaku tuk hilangkan kelelahannya..
Jika Engkau ciptakan raganya
jadikanlah tulang rusuk kiriku tuk temani kesendiriannya..
[Claudia]

Jumat, 12 Oktober 2012

Hanya Ingin Kau Tahu, Ukhti..

Sering aku bertanya
Apa bisa aku merangkulmu, ukhti? 
Memang sedikit takut membuatmu tersinggung 
takut membuatmu sakit hati karena perkataanku 
atau pula ajakanku 

Tapi, tahukah ukhti.. 
malah akulah yang lebih tersinggung dan sakit hati 
ketika kau mengacuhkan perkataanku 
ketika pula kau mengabaikan ajakanku 

Mungkin ini namanya perjuangan dakwah 
Kesabaran harusku miliki 
Keberanian harusku sanggupi 
Keistiqomahan harusku genggami 

Perlu kau ketahui, ukh.. 
Aku hanya ingin kita bisa terus bersama dalam 
dekapan cahaya-Nya 
Agar kita bisa terus bersama di dunia dan di akhirat kelak 
dengan ridho-Nya 
Karena aku sangat menyayangimu, ukhti
[Claudia